RESUMO
Objective: The present study was aimed to determine the cytotoxicity concentration (CTC50) of different extracts made from the leaf and stem bark of an ethno botanically selected S. pubescens against Human liver carcinoma (Hep G2), Human colon carcinoma (CaCo2) and Human breast cancer (T-47 D) cell lines. Methods: Ethnobotanical survey was done through interviewing traditional medicinal practitioners then a potential herbal plant was selected after a thorough literature survey and its identity was confirmed. The soxhlet extraction method was adopted using five different solvents from leaf and stem bark powders of the study plant and the CTC50 of all the extracts were determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay method. Results: Among the different extracts, CTC50 values were significant for stem bark extracts made from methanol (24.51±0.08 µg/ml) against Hep G2, while leaf chloroform extract was promising (57.15±1.75 µg/ml) against CaCo2 and n-hexane extract of leaf exhibited significant value (20.27±1.52 µg/ml) against T-47 D cancer cell lines. Conclusion: The major findings of the present study clearly provides evidence that the leaf and stem bark of S. pubescens possesses the potential anticancer bioactive compound solasodine.
RESUMO
The aim of the present study is to carry out the quantification of Clerodendrum serratum containing poly herbal formulations by HPTLC and to study the invitro anti-oxidant activity of nitric oxide scavenging activity of the roots of Clerodendrum serratum.The rhizome part of Clerodendrum serratum has been selected, dried, powdered and extracted with petroleum ether, chloroform, ethyl acetate and methanol successively by continuous hot percolation method using soxhlet apparatus. Preliminary phytochemical screening of the successive extracts showed the presence of chemical constituents like triterpenoids, flavonoids, alkaloids, tannins, glycosides and sesquiterpenes. The Rf value of stigmasterol was found to be 0.80 ± 0.02.The developed methods were validated as per ICH guidelines. The LOD and LOQ was found to be 100 and 300 ng/spot respectively for HPTLC. The anti-oxidant activity was highest in petroleum ether extract when compared to the other extracts by using ascorbic acid as control. This shows that the polyherbal formulation containing stigmasterol has medicinal value and that it may be utillised for its anti-oxidant properties.